Limited Proteolysis of Cytoplasmic and Nuclear Uterine Estradiol Receptors Yields Identical Estradiol-Binding Fragments
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منابع مشابه
Specificity of oligodeoxynucleotide binding of mouse uterine cytosol estradiol receptors.
The relative capacities of oligodeoxynucleotides, covalently linked to cellulose, to bind estradiol receptor complexes (E2R) of mouse uterine cytosol have been shown to follow the order oligo(dG) > oligo(dT) greater than or equal to oligo(dC) > oligo(dA). The E2R . oligo(dT)-cellulose-binding reaction is more sensitive to Cibacron blue F3GA than is E2R . oligo(dG)-cellulose or oligo(dC)-cellulo...
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We have previously reported that estradiol enters uterine cell suspensions by passive diffusion (Miiller, R. E., Traish, A. M., and Wotiz, H. H. (1977) The Endocrine Society Program and Abstracts, 59th Annual Meeting Abstr. 48; Miiller, R. E., Johnston, T. C., Traish, A. M., and Wotiz, H. H. (1979) in Steroid Hormone Receptor Systems (Leavitt, W. W., and Clark, J. C., eds) Plenum Press, New Yor...
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A cell-free system was established to study the role of steroid hormones in transc:riptional control. The system consists of hormone-receptorchromatin complex formation ustng partially purified receptor and chromatin. Micrococcal nuclease digestion of the complex released a 7S form of receptor. The absorbance at 260 nm also showed a peak in the 7S region of the sucrose gradient. DNAase I digest...
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The binding of estradiol to nuclear fractions extracted from human breast carcinomatous tissue was demonstrated. The material, which was extracted with KCI, had an approximate molecular weight of 37,000 and bound estradiol with both high and low affinity (K^ = 1 nM, type A receptors; K¿& 30 nw, type B receptors) as calculated according to the method of Scatchard. Competition studies indicated ...
متن کاملNuclear estradiol-binding sites in human breast cancer.
The binding of estradiol to nuclear fractions extracted from human breast carcinomatous tissue was demonstrated. The material, which was extracted with KCl, had an approximate molecular weight of 37,000 and bound estradiol with both high and low affinity (Kd congruent to 1 nM, type A receptors; Kd congruent to 30 nM, type B receptors) as calculated according to the method of Scatchard. Competit...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1976
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1976.tb10522.x